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| Registros recuperados: 46 | |
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| Lapegue, Sylvie; Diaz-almela, E.; Launey, Sophie; Ledu, Christophe; Boudry, Pierre; Naciri-graven, Yamama; Bonhomme, Francois. |
| Natural populations of the European flat oyster, Ostrea edulis, have been harvested since Roman times. This species is distributed along the European Atlantic coasts, from Norway to Morocco, as well as the Mediterranean Sea and Black sea coasts. Previous studies, based on allozymes, have concluded that isolation by distance is taking place in the Mediterranean sea and the Atlantic ocean. Because of the presumed non neutrality of these markers, another approach was made to examine the genetic differentiation of this species using nuclear microsatellite and mitochondrial markers, both considered as neutral, and to compare the results to those observed with allozymes. |
| Tipo: Text |
Palavras-chave: Microsatellite markers; Ostrea edulis; Flat oyster; Genetic. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/acte-3497.pdf |
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| Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois. |
| Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of Crassostrea gigas, the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each... |
| Tipo: Text |
Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf |
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| Vonau, Vincent; Rouxel, Catherine; Saulnier, Denis; Cochennec-laureau, Nathalie; Nedelec, Georges; Goyard, Emmanuel. |
| The tropical seabass Lates calcarifer was introduced in Tahiti in 1984 and then was domesticated without other introduction. Three successive generations have been obtained in captivity. Tissue samples of the 38 animaIs which represent the total Tahitian broodstock were preserved in alcohol to be genotyped with four microsatellite markers (Yue et al., 2001). Three of four described markers were successfully transferred to the laboratory of genetics of Tahiti (marker LcaM01, LcaM02, LcaM03), as the revelation technology available locally did not allow the use of LcaM04 in routine. The results show that the genetic diversity of the tahitian broodstock is not equivalent of the one of the species, which could be explained by the small size of the founder... |
| Tipo: Text |
Palavras-chave: Lates calcarifer; Marqueurs microsatellites; Reproducteurs; Variabilité génétique; Lates calcarifer; Microsatellite markers; Breeding stock; Genetic diversity. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00142/25300/23372.pdf |
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| Santos,Silvia Correa; Ruggiero,Carlos; Silva,Cristina Lacerda Soares Petrarolha; Lemos,Eliana Gertrudes Macedo. |
| In experimental areas of the Education and Researches Ilha Solteira and Jaboticabal UNESP/Campus farms were selected and tagged 20 hermaphrodite plants and 20 feminine of cultivar Sunrise Solo, Improved Sunrise Solo cv.72/12 and Baixinho of Santa Amália.The seeds origined of the selected fruits were cropped to be analysed the self-pollination efficiency and frequency of the sex in the progenies. After that, samples of the young leaf of the matrix plants were colected for the extration of the DNA. It was built five library enriched of microsatellite sequencies, using probes (TCA)10, (TC)13, (GATA)4, (CAC)10 e (TGAG)8.It was possible the development of the primers only in the library that has utilized the probe (TCA)10. This probe allowed the design of 32... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya; Sex; Microsatellite markers; Library. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000200020 |
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| Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno. |
| To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained in diploids using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. Recently, in the U.S.A., Australia and New Zealand, countries where hatcheries are a major source of C. gigas juveniles, family-based selective... |
| Tipo: Text |
Palavras-chave: Microsatellite markers; Selective breeding; Genetic; Genetic variability; Crassostrea gigas; Pacific oysters. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/acte-3449.pdf |
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| Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre. |
| The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to persue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period, (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females, (3) the variance of individual... |
| Tipo: Text |
Palavras-chave: Recruitment; Microsatellite markers; Ostrea edulis; European flat oyster; Genetic. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/acte-3485.pdf |
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| Boudry, Pierre. |
| To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the production of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or other traits, could be obtained in diploids. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. In the U.S.A., Australia and New Zealand, family-based selective breeding programs have recently been initiated to improve growth, disease tolerance and yield. In Europe, where both natural and... |
| Tipo: Text |
Palavras-chave: Microsatellite markers; Crassostrea gigas; Selective breeding; Triploid; Pacific oyster; Genetic. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3451.pdf |
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| Silva,Kleibe de Moraes; Paixão,Débora Martins; Silva,Priscila Vendramini; Solero,Bruna Pena; Pereira,Mario Sérgio; Lopes,Paulo Sávio; Guimarães,Simone Eliza Facioni. |
| The objective was to map QTL on porcine chromosome 4 and to associate them with carcass and internal organ traits in an F2 population. The F1 population was produced by outbreed crossing, using two native Brazilian breed Piau boars and 18 commercial sows. A total of 617 F2 animals issued from 11 F1 boars and 54 F1 sows were typed for a total of five microsatellite markers. The data were analyzed by multiple regressions developed for the analysis of crosses between outbred lines, using the QTL Express software. Significant evidence for QTL was found for pig chromosome 4 regarding carcass and internal organ traits. All QTL were detected in the same region of the chromosome, designated FAT1. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fatness; Pig production; Divergent crosses; Microsatellite markers; QTL mapping. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300013 |
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| Kuldeep,Tyagi; Nandan,Rajiv; Kumar,Uttam; Prasad,Lal Chand; Chand,Ramesh; Joshi,Arun Kumar. |
| Spot blotch resistant (IBON 18) and susceptible (RD 2508) lines were crossed to investigate inheritance of resistance and to identify simple sequence repeats (SSRs) associated with resistance. F1 resistance was intermediate and suggested additive nature of inheritance. Three additive genes was noted in the distribution of F3, F4 and F5 generations. In F6 and F6-7, the quantitative and qualitative approaches also suggested the control of three resistance genes. The parents and the RILs (F6/F6-7) were grown in four environments and spot blotch severity recorded. Forty five SSR primers, specific for chromosomes 1 (7H) and 5 (1H), were applied. Of these, 12 were polymorphic between the parents, and between the resistant and susceptible bulks. Three markers BMS... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellite markers; Molecular marker; Spot blotch; Cochliobolus sativus; Barley. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400021 |
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| Reis,Sávio P.; Gonçalves,Evonnildo C.; Silva,Artur; Schneider,Maria P.C.. |
| In this study, 15 microsatellite DNA loci used in comparative tests by the International Society for Animal Genetics were applied to the evaluation of genetic diversity and management, and the efficiency of paternity testing in Marajoara horses and Puruca ponies from the Marajó Archipelago. Based on the genotyping of 93 animals, mean allelic diversity was estimated as 9.14 and 7.00 for the Marajoara and Puruca breeds, respectively. While these values are similar to those recorded in most European breeds, mean levels of heterozygosity were much lower (Marajoara 49%, Puruca 40%), probably as a result of high levels of inbreeding in the Marajó populations. The mean informative polymorphic content of this 15-marker system was over 50% in both breeds, and was... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Marajoara horses; Puruca ponies; Microsatellite markers; Genetic diversity; Geneaological control. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100014 |
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| Paixão,Débora M.; Silva Filho,Miguel I. da; Pereira,Mário S.; Lopes,Marcos S.; Barbosa,Leandro; Souza,Katiene Régia Silva; Lopes,Paulo S.; Guimarães,Simone E.F.. |
| The objective of this study was to map quantitative trait loci (QTL) on porcine chromosomes 16, 17 and 18 and to determine their association with carcass, organ and meat quality traits. An F2 population was produced by crossing two boars of the naturalized Brazilian Piau breed with 18 commercial females (Landrace x Large White x Pietrain). The population was genotyped for 11 microsatellite markers distributed over the three chromosomes and the results were used to construct a marker-specific linkage map for the population. Analysis of the polymorphic information content showed that the microsatellite markers were adequate for the study of quantitative traits. QTL were identified by regression interval mapping using QTL Express software. QTL not previously... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Divergent crosses; Microsatellite markers; Pig production; QTL mapping. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500016 |
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| Ambo,Marcel; Campos,Raquel de Lello Rocha; Moura,Ana Sílvia Meira Tavares; Boschiero,Clarissa; Rosário,Millor Fernandes do; Ledur,Mônica Corrêa; Nones,Kátia; Coutinho,Luiz Lehmann. |
| A linkage map is essential not only for quantitative trait loci (QTL) mapping, but also for the organization and location of genes along the chromosomes. The present study is part of a project whose major objective is, besides from construction the linkage maps, the whole genome scan for mapping QTL for performance traits in the Brazilian experimental chicken population. Linkage maps of chicken chromosomes 6 to 8, 11 and 13 were constructed based on this population. The population was developed from two generations of crossbreeding between a broiler and a layer line. Fifty-one microsatellite markers were tested, from which 28 were informative: 4, 8, 7, 4 and 5 for chromosomes 6, 7, 8, 11 and 13, respectively. A SNP located in the leptin receptor gene was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gallus gallus; Quantitative trait loci (QTL); Genetic maps; Microsatellite markers; Animal breeding. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500001 |
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| Missio,Robson Fernando; Caixeta,Eveline Teixeira; Zambolim,Eunize Maciel; Pena,Guilherme Ferreira; Ribeiro,Ana Paula; Zambolim,Laércio; Pereira,Antônio Alves; Sakiyama,Ney Sussumu. |
| EST-SSR markers were used to investigate the genetic diversity among and within coffee populations, to explore the possibility of their use for fingerprinting of cultivars and to assist breeding programs. Seventeen markers, developed from ESTs (Expressed Sequence Tags) from the Brazilian Coffee Genome Project, were used. All markers showed polymorphism among the genotypes assessed. The average number of allele per primer was 5.1. The highest polymorphisms were found within C. canephora (88.2%) and rust-resistant varieties (35.3%). About 29.4% of the markers differentiated C. arabica from Híbrido de Timor; it was also possible to identify those closest and farthest from C. arabica . The analysis of population-grouped genotypes revealed a 64.0% genetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA markers; Coffea sp.; UPGMA; Amova; Microsatellite markers. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052009000300003 |
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| Registros recuperados: 46 | |
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